Angebote zu "Insulin" (4 Treffer)

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Genetic Engineering and Molecular Studies of In...
40,99 € *
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This book reveals some details of research done at Marshall University, USA, involving functional aspects of pancreatic beta cells in several experimental models: avian, transgenic mice, and beta-cell lines. In this book you will gain some knowledge of genetic engineering approaches to improve beta-cell insulin secretion which could be applied to manage models and cases of diabetes mellitus. This work is presented as three separate chapters. The first chapter aims to gain an understanding of the expression pattern of CaBP28k in the chicken pancreas and to explore its spatial relationships with insulin in beta cells. The second chapter is to identify the influence of CaBP28k-ablation on the dynamics of [Ca2+]i in response to glucose stimulation in murine beta cells of CaBP28k-KO mice pancreatic islets and wildtype islets. The third chapter is an attempt to elucidate the genomic and non-genomic effects of in vitro CaBP28k-ablation in a cultured beta-cell line. It highlights the effects on the gene expression profile and insulin secretory responses from cultured rat insulinoma cell lines using antisense oligonucleotides transfection.

Anbieter: buecher
Stand: 26.05.2020
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Genetic Engineering and Molecular Studies of In...
59,00 € *
ggf. zzgl. Versand

This book reveals some details of research done at Marshall University, USA, involving functional aspects of pancreatic beta cells in several experimental models: avian, transgenic mice, and beta-cell lines. In this book you will gain some knowledge of genetic engineering approaches to improve beta-cell insulin secretion which could be applied to manage models and cases of diabetes mellitus. This work is presented as three separate chapters. The first chapter aims to gain an understanding of the expression pattern of CaBP28k in the chicken pancreas and to explore its spatial relationships with insulin in beta cells. The second chapter is to identify the influence of CaBP28k-ablation on the dynamics of [Ca2+]i in response to glucose stimulation in murine beta cells of CaBP28k-KO mice pancreatic islets and wildtype islets. The third chapter is an attempt to elucidate the genomic and non-genomic effects of in vitro CaBP28k-ablation in a cultured beta-cell line. It highlights the effects on the gene expression profile and insulin secretory responses from cultured rat insulinoma cell lines using antisense oligonucleotides transfection.

Anbieter: Dodax
Stand: 26.05.2020
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Regulation of Insulin Biosynthesis
59,00 € *
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Intracellular signaling by which pancreatic beta- cells synthesize and secrete insulin in control of glucose homeostasis is not fully understood. Here we show that Shp2, a cytoplasmic tyrosine phosphatase possessing 2 SH2 domains, coordinates signaling events required for insulin biosynthesis in beta-cells. Mice with conditional ablation of the Shp2/Ptpn11 gene in the pancreas exhibited defective glucosestimulated insulin secretion and impaired glucose tolerance. Consistently, siRNA-mediated Shp2-knockdown in rat insulinoma INS-1 832/13 cells resulted in decreased insulin production and secretion despite an increase in cellular ATP. Shp2 modulates the strength of signals flowing through Akt/FoxO1 and Erk pathways, culminating in control of Pdx1 expression and activity on Ins1 and Ins2 promoters, and forced Pdx1 expression rescued insulin production in Shp2-knockdown beta-cells. Therefore, Shp2 acts as a signal coordinator in beta-cells, orchestrating multiple pathways controlling insulin biosynthesis to maintain glucose homeostasis.

Anbieter: Dodax
Stand: 26.05.2020
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